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KMID : 0364820140500040360
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Korean Journal of Microbiology
2014 Volume.50 No. 4 p.360 ~ p.367
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Antifungal Activity of Rheum undulatum on Candida albicans by the Changes in Membrane Permeability
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Lee Heung-Shick
Kim Yeon-Hee
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Abstract
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Candida albicans is an opportunistic and the most prevalent fungal pathogen that can cause superficial and systemic
infections in immunocompromised patients. C. albicans can promote the transition from budding yeast to
filamentous form, generating biofilms. Infections associated with C. albicans biofilms are frequently resistant to
conventional antifungal therapy. Therefore, the development of more effective antifungal drugs related with biofilm
formation is required urgently. The roots of Rheum undulatum have been used for medicinal purposes in Korea and
China traditionally. The aim of present study was to evaluate the effect of R. undulatum extract upon preformed
biofilms of 12 clinical C. albicans isolates and the antifungal activities. Its effect on preformed biofilms was
evaluated using XTT reduction assay, and metabolic activity of all tested strains was reduced significantly (49.4 ±
6.0%) at 0.098 mg/ml R. undulatum. The R. undulatum extract blocked the adhesion of C. albicans biofilms to
polystyrene surfaces, and damaged the cell membrane integrity of C. albicans which was analyzed by CFDA, AM,
and propidium iodide double staining. It caused cell lysis which was observed by Confocal laser scanning and phase
contrast microscope after propidium iodide and neutral red staining, respectively. Membrane permeability was
changed as evidenced by crystal violet uptake. The data suggest that R. undulatum inhibits biofilm formation by C.
albicans, which can be associated with the damage of the cell membrane integrity, the changes in the membrane
permeability and the cell lysis of C. albicans.
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KEYWORD
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Candida albicans, Rheum undulatum, antifungal activity, biofilm, membrane permeability
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